Document Type : Original Article

Authors

• Maryam Alsadat Hashemipour ¹
• Hoda Mehrabizadeh Honarmand ²
• Farideh Falsafi ³
• Mehrnaz Tahmasebi Arashlo ³
• Saied Rajabalian ⁴
• Sayed Amir Hossein Gandjalikhan Nassab ⁵

¹ Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.

² Resident of Oral and Maxillofacial Surgery and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.

³ Dentist, Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.

⁴ Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

⁵ Medical Student, Kerman Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

Statement of the Problem: Use of cyclooxygenase inhibitors as chemotherapy agents has attracted the attention of a large number of investigators in recent years. Given the importance of cancer therapy, only a limited number of studies have been carried out to investigate the effects of cyclooxygenase inhibitors on specific cell lines.Purpose: This research aimed to determine the in vitro cytotoxic effects of cyclooxygenase inhibitors (COX-1 and COX-2 inhibitors) on KB, Saos-2, 1321N, U-87MG, SFBF-PI 39 cell lines.Materials and Method: Powders of celecoxib, mefenamic acid, aspirin and indometacin were dissolved in the appropriate solvent. The viability of cell lines was carried out by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay technique. Data gathered from four separate experiments were expressed as mean±SD. Statistical significance was defined at p< 0.05 by using analysis of variance. Significant treatment mean values were subjected to post-hoc Tukey’s test.Results: Celecoxib showed marked cytotoxic effects on KB, Saos-2, and 1321N cells, which was significant in comparison with the control group. Celecoxib was not effective in killing U-87MG cell line. Mefenamic acid exerted cytotoxic effects on KB, Saos-2, and 1321N cells, where the viability was approximately 75%. U-87MG cells were resistant to mefenamic acid. Indometacin had the highest rate of activity on U-87MG cells, which was significant in comparison with the control group. Aspirin did not exhibit any activity on these cell lines and was not effective in killing U-87MG, KB, Saos-2, and 1321N cells.Conclusion: This research showed that celecoxib, indometacin, and mefenamic acid have the cytotoxic effects on KB, Saos-2, 1321N and U-87MG cell lines. Therefore, it appears that these drugs can be considered as anti-neoplastic agents in the experimental phase.Keywords ● In Vitro  ● Cytotoxicity  ● Drug, Celecoxib  ● Mefenamic Acid  ● Aspirin  ● Indometacin  ● Cell lines