Document Type: Original Article

Authors

1 Post Graduate Student, Dept. of Oral Medicine, School of Dentistry, Shiraz University of Medical sciences, Shiraz, Iran.

2 Dept. of Oral Medicine, School of Dentistry, Shiraz University of Medical sciences, Shiraz, Iran.

3 Dept. of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

4 Dept. of Oral and Maxillofacial Pathology, Prevention and Treatment of Oral and Dental Diseases Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

5 Transgenic Technologies Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

6 School of Dentistry, University of Southern California, Los Angeles, USA.

7 Dept. of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

8 Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

9 Laboratory Animals Center, Shiraz University of Medical Sciences, Shiraz, Iran.

10 Dept. of Pharmacology, School of Medicine, Transgenic Technologies Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Statement of the Problem: Oral mucositis (OM) is a common side effect of anti-cancer drugs and needs significant attention for its prevention.Purpose: This study aimed to evaluate the healing effects of olive leaf extract on 5-fluorouracil-induced OM in golden hamster.Materials and Method: OM was induced in 63 male golden hamsters by the combination of 5-fluorouracil injections (days 0, 5 and 10) and the abrasion of the cheek pouch (days 3 and 4). On day 12, hamsters were received topical olive leaf extract ointment, base of ointment, or no treatment (control) for 5 days. Histopathology evaluations, blood examinations, and tissue malondialdehyde level measurement were performed 1, 3 and 5 days after treatments.Results: Histopathology score and tissue malondialdehyde level were significantly lower in olive leaf extract treated group in comparison with control and base groups (p= 0.000). Significant decreases in white blood cell, hemoglobin, hematocrit , and mean corpuscular volume and an increase in mean corpuscular hemoglobin concentration were observed in olive leaf extract treated group in comparison with control and base groups (p< 0.05).Conclusion: Our findings demonstrated that daily application of olive leaf extract ointment had healing effect on 5-fluorouracil induced OM in hamsters. Moreover, the beneficial effect of olive leaf extract on OM might be due to its antioxidant and anti-inflammatory properties.Keywords ● 5- fluorouracil  ● Anti-inflammatory  ● Antioxidant  ● Olive Leaf  ● Oral Mucositis