Document Type: Original Article


Dept. Endodontic, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.


Statement of the Problem: The maintenance of viable periodontal ligament cells is the most important issue in the long-term preservation of avulsed teeth.Purpose: The aim of this study was to assess aloe vera as a new storage media in maintaining the cell viability of dry-stored teeth in comparison with soy milk, Hank`s balanced salt solution (HBSS), and milk.Materials and Method: Twenty one extracted dog premolar teeth were dried for 30 minutes and stored in soy milk, HBSS, milk, and aloe vera extract (50%) for 45 minutes (n=6 for each). Furthermore, positive and two negative control groups (n=6), corresponding to 0 min, 30 min, and 2-hour drying times were also prepared respectively. The number of viable cells was counted following storage using Trypan blue exclusion. Data were statistically analyzed using the one-way ANOVA and post hoc Tukey-HSD test.Results: Statistical analysis showed no significant differences in cell viability among aloe vera, soymilk, and HBSS- stored teeth; however, they were all superior to milk.Conclusion: Aloe vera extract can be recommended as a suitable storage media for avulsed teeth.Keywords ● Aloe vera  ● Cell viability  ● Periodontal ligament  ● Soy milk  ● Tooth avulsion