Document Type : Original Article

Authors

1 Dept. of Oral and Maxillofacial Pathology, Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Student's Research Committee, School of Dentistry, International Branch, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Dept. of Periodontics, Faculty of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.

4 Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Statement of the Problem: Human tooth is clinically the most appropriate material that can be used for in-vitro dental research. However, there are limitations and drawbacks for using human teeth. Therefore, alternatives to samples of human teeth for dental studies are necessary.
Purpose: This study purposed to evaluate and compare the microstructure and chemical composition of enamel and dentin of teeth in some lab animals.
Materials and Method:In this experimental study, teeth of mouse, rabbit, guinea pig, dog, cat, and sheep were used. Scanning electron microscope observations and X-ray diffraction analysis were performed on samples.
Results: This study revealed resemblance in general structure of dentin and enamel between mentioned animals and human. The minimum mean of dentinal tubules diameter is found in guinea pig (0.5µ), while the highest is in cat (1.5µ). Also the lowest and the highest mean intertubular distance was measured respectively in guinea pig (3µ) and sheep (4.8µ) and the maximum and minimum mean diameter of rods was measured in rabbit (6.6µ) and guinea pig (1.5µ), respectively.
Conclusion: The recorded details and the measured values indicate great resemblance between dog and human dentin and enamel. Cat is in the second place for dentinal studies; sheep and guinea pig have the least resemblance to human within the scope of the reviewed criteria.

Keywords

1. Yassen GH, Platt JA, Hara AT. Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci. 2011; 53: 273–282. [PubMed[Google Scholar]
2. Laurance-Young P, Bozec L, Gracia L, Rees G, Lippert F, Lynch RJ, et al. A review of the structure of human and bovine dental hard tissues and their physicochemical behaviour in relation to erosive challenge and remineralisation. J Dent. 2011; 39: 266–272. [PubMed[Google Scholar]
3. Sano H, Ciucchi B, Matthews WG, Pashley DH. Tensile properties of mineralized and demineralized human and bovine dentin. J Dent Res. 1994; 73: 1205–1211. [PubMed[Google Scholar]
4. Schilke R, Lisson JA, Bauss O, Geurtsen W. Comparison of the number and diameter of dentinal tubules in human and bovinedentine by scanning electron microscopic investigation. Arch Oral Biol. 2000; 45: 355–361. [PubMed[Google Scholar]
5. Almeida KG, Scheibe KG, Oliveira AE, Alves CM, Costa JF. Influence of human and bovine substrate on the microleakage of two adhesive systems. J Appl Oral Sci. 2009; 17: 92–96. [PMC free article] [PubMed[Google Scholar]
6. Turssi CP, Messias DF, Corona SM, Serra MC. Viability of using enamel and dentin from bovine origin as a substitute for human counter-parts in an intraoral erosion model. Braz Dent J. 2010; 21: 332–336. [PubMed[Google Scholar]
7. Popowics TE, Rensberger JM, Herring SW. Enamel microstructure and microstrain in the fracture of human and pig molar cusps. Arch Oral Biol. 2004; 49: 595–605. [PubMed[Google Scholar]
8. Lopes FM, Markarian RA, Sendyk CL, Duarte CP, Arana-Chavez VE. Swine teeth as potential substitutes for in vitro studies in tooth adhesion: a SEM observation. Arch Oral Biol. 2006; 51: 548–551. [PubMed[Google Scholar]
9. Edmunds DH, Whittaker DK, Green RM. Suitability of human, bovine, equine, and ovine tooth enamel for studies of artificial bacterial carious lesions. Caries Res. 1988; 22: 327–336. [PubMed[Google Scholar]
10. Takagi S, Liao H, Chow LC. Effect of tooth-bound fluoride on enamel demineralization/ remineralization in vitro. Caries Res. 2000; 34: 281–288. [PubMed[Google Scholar]
11. Zoba HA, Rabab M. Histomorphological Study of Dentine Pulp Complex of Continuously Growing Teeth in the. Rabbits. Life Sci J. 2012; 9: 1554–1564. [Google Scholar]
12. Møinichen CB, Lyngstadaas SP, Risnes S. Morphological characteristics of mouse incisor enamel. J Anat. 1996; 189 (Pt 2): 325–333. [PMC free article] [PubMed[Google Scholar]
13. Nanci A. Ten Cate's oral histology: Development, structure and function. 8th ed. St Louis, Missouri: Mosby, Elsevier; 2008. pp. 176–180. [Google Scholar]
14. Skobe Z, Prostak KS, Trombly PL. Scanning electron microscope study of cat and dog enamel structure. J Morphol. 1985; 184: 195–203. [PubMed[Google Scholar]
15. Xue J, Zavgorodniy AV, Kennedy BJ, Swain MV, Li W. X-ray microdiffraction, TEM characterization and texture analysis of human dentin and enamel. J Microsc. 2013; 251: 144–153. [PubMed[Google Scholar]