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Effect of Two Methods of Remineralization and Resin Infiltration on Surface Hardness of Artificially Induced Enamel Lesions

Document Type : Original Article

Authors

1 Dentist

2 Dental Material Research Center of Tehran University of Medical Sciences, Dept. of Restorative and Cosmetic Dentistry, Islamic Azad University, Tehran, Iran.

3 Dept. of Restorative and Cosmetic Dentistry, Islamic Azad University, Tehran, Iran.

4 Dept. of Restorative and Cosmetic Dentistry, Semnan University of Medical Sciences, Semnan, Iran.

5 Dental Research Center, Dental Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Statement of the Problem: The progression of incipient carious lesions may be simply prevented by non-invasive remineralization of lesions, eliminating the need for invasive and high-cost restorative procedures.
Purpose: This study aimed to assess the effect of two commonly used remineralizing agents and resin infiltration on surface micro hardness of incipient enamel lesions at different time points.
Materials and Method: In this in vitro study 45 intact human maxillary central incisors were selected. After disinfection, enamel samples measuring 5x5x2.5 mm were cut out of the labial surface of the teeth. The surface of samples was polished and they were mounted in auto-polymerizing acrylic resin. According to Amaechi’s method samples were immersed in acidified hydroxyethylcellulose system (pH = 4.5) for 96 hours to induce white spot lesions (WSLs). The baseline surface micro hardness of samples was measured using a Vickers hardness tester, then the samples were randomly divided into three groups (n=15) based on different remineralization methods: MI-Paste Plus, Remin Pro and ICON-Infiltrant according to the manufacturer’s instructions. All samples were stored in anti-dry mouth treatment agent during the experiment and their surface hardness was measured at 15 days (T1) and 20 weeks (T2).  
Results: The hardness of samples in MI-Paste Plus and Remin Pro groups significantly increased at both T1 and T2 (p < 0.001) but this increase was not significant in ICON group (p > 0.05).
Conclusion: MI-Paste Plus and Remin Pro can efficiently increase the hardness of incipient enamel lesions.

Keywords

References
1. Kutsch VK. Dental caries: an updated medical model of risk assessment. J Prosthet Dent. 2014; 111: 280–285. [PubMed] [Google Scholar]
2. Johansson I, Holgerson PL, Kressin NR, Nunn ME, Tanner AC. Snacking habits and caries in young children. Caries Res. 2010; 44: 421–430. [PMC free article] [PubMed] [Google Scholar]
3. Wang Y, Mei L, Gong L, Li J, He S, Ji Y, et al. Remineralization of early enamel caries lesions using different bioactive elements containing toothpastes: An in vitro study. Technol Health Care. 2016; 24: 701–711. [PubMed] [Google Scholar]
4. Swarup JS, Arathi R. Enamel Surface Remineralization: Using Synthetic Nanohydroxyapatite. Contemp Clin Dent. 2012; 3:433–436. [PMC free article] [PubMed] [Google Scholar]
5. Gao SS, Zhang S, Mei ML, Lo ECM, Chu CH. Caries remineralisation and arresting effect in children by professionally applied fluoride treatment – a systematic review. BMC Oral Health. 2016; 16:12. [PMC free article] [PubMed] [Google Scholar]
6. Senestraro SV, Crowe JJ, Wang M, Vo A, Huang G, Ferracane J, et al. Minimally invasive resin infiltration of arrested white-spot lesions: a randomized clinical trial. J Am Dent Assoc. 2013; 144: 997–1005. [PubMed] [Google Scholar]
7. Pitts N, Amaechi B, Niederman R, Acevedo AM, Vianna R, Ganss G, et al. Global Oral Health Inequalities: Dental Caries Task Group—Research Agenda. Adv Dent Res. 2011; 23:211–220. [PMC free article] [PubMed] [Google Scholar]
8. Huang GJ, Roloff-Chiang B, Mills BE, Shalchi S, Spiekerman C, Korpak AM, et al. Effectiveness of MI Paste Plus and PreviDent fluoride varnish for treatment of white spot lesions: a randomized controlled trial. Am J Orthod Dentofacial Orthop. 2013; 143: 31–41. [PMC free article] [PubMed] [Google Scholar]
9. Sadr Haghighi H, Skandarinejad M, Abdollahi AA. Laser Application in Prevention of Demineralization in Orthodontic Treatment. J Lasers Med Sci. 2013; 4: 107–110. [PMC free article] [PubMed] [Google Scholar]
10. Guclu ZA, Alacam A, Coleman NJ. A 12-Week Assessment of the Treatment of White Spot Lesions with CPP-ACP Paste and/or Fluoride Varnish. Biomed Res Int. 2016; 2016:8357621. [PMC free article] [PubMed] [Google Scholar]
11. Heravi F, Ahrari F, Tanbakuchi B. Effectiveness of MI Paste Plus and Remin Pro on remineralization and color improvement of postorthodontic white spot lesions. Dent Res J (Isfahan) 2018; 15: 95–103. [PMC free article] [PubMed] [Google Scholar]
12. Ou XY, Zhao YH, Ci XK, Zeng LW. Masking white spots of enamel in caries lesions with a non-invasive infiltration technique in vitro. Genet Mol Res. 2014; 13: 6912–6919. [PubMed] [Google Scholar]
13. Bakry AS, Abbassy MA. Increasing the efficiency of CPP-ACP to remineralize enamel white spot lesions. J Dent. 2018; 76: 52–57. [PubMed] [Google Scholar]
14. Amaechi BT, Higham SM, Edgar WM. Factors affecting the development of carious lesions in bovine teeth in vitro. Arch Oral Biol. 1998; 43: 619–628. [PubMed] [Google Scholar]
15. Heshmat H, Ganjkar MH, Miri Y, Fard MJ. The effect of two remineralizing agents and natural saliva on bleached enamel hardness. Dent Res J (Isfahan) 2016; 13: 52–57. [PMC free article] [PubMed] [Google Scholar]
16. Salehzadeh Esfahani K, Mazaheri R, Pishevar L. Effects of Treatment with Various Remineralizing Agents on the Microhardness of Demineralized Enamel Surface. J Dent Res Dent Clin Dent Prospects. 2015; 9: 239–245. [PMC free article] [PubMed] [Google Scholar]
17. Borges BC, de Souza Borges J, de Araujo LS, Machado CT, Dos Santos AJ, de Assuncao Pinheiro IV. Update on nonsurgical, ultraconservative approaches to treat effectively non-cavitated caries lesions in permanent teeth. Eur J Dent. 2011; 5: 229–236. [PMC free article] [PubMed] [Google Scholar]
18. Wu G, Liu X, Hou Y. Analysis of the effect of CPP-ACP tooth mousse on enamel remineralization by circularly polarized images. Angle Orthod. 2010; 80: 933–938. [PubMed] [Google Scholar]
19. Vyavhare S, Sharma DS, Kulkarni VK. Effect of three different pastes on remineralization of initial enamel lesion: an in vitro study. J Clin Pediatr Dent. 2015; 39: 149–160. [PubMed] [Google Scholar]
20. Jayarajan J, Janardhanam P, Jayakumar P, Deepika Efficacy of CPP-ACP and CPP-ACPF on enamel remineralization - an in vitro study using scanning electron microscope and DIAGNOdent. Indian J Dent Res. 2011; 22: 77–82. [PubMed] [Google Scholar]
21. Leila B, Nemati S, Neda H, Khanehmasjedi M. The Effect of MIpaste Plus and Reminpro on Incipient Caries Using DIAGNOdent and SEM: An Invitro Study. J Natl Med Assoc. 2017; 109: 192–197. [PubMed] [Google Scholar]
22. Oliveira GM, Ritter AV, Heymann HO, Swift E Jr, Donovan T, Brock G. Remineralization effect of CPP-ACP and fluoride for white spot lesions in vitro. J Dent. 2014; 42: 1592–1602. [PMC free article] [PubMed] [Google Scholar]
23. Alencar CRB, Oliveira GC, Magalhães AC, Buzalaf MAR, Machado MAAM, Honório HM. In situ effect of CPP-ACP chewing gum upon erosive enamel loss. J Appl Oral Sci. 2017; 25: 258–264. [PMC free article] [PubMed] [Google Scholar]
24. Kargul B, Altinok B, Welbury R. The effect of casein phosphopeptide-amorphous calcium phosphate on enamel surface rehardening. An in vitro study. Eur J Paediatr Dent . 2012; 13:123–127. [PubMed] [Google Scholar]
25. Chen H, Liu X, Dai J, Jiang Z, Guo T, Ding Y. Effect of remineralizing agents on white spot lesions after orthodontic treatment: a systematic review. Am J Orthod Dentofacial Orthop. 2013; 143: 376–382. [PubMed] [Google Scholar]
26. Kamath U, Sheth H, Mullur D, Soubhagya M. The effect of Remin Pro(R) on bleached enamel hardness: an in-vitro study. Indian J Dent Res. 2013; 24: 690–693. [PubMed] [Google Scholar]
27. Shetty S, Hegde MN, Bopanna TP. Enamel remineralization assessment after treatment with three different remineralizing agents using surface micro hardness: An in vitro study. J Conserv Dent. 2014; 17: 49–52. [PMC free article] [PubMed] [Google Scholar]
28. Paris S, Hopfenmuller W, Meyer-Lueckel H. Resin infiltration of caries lesions: An efficacy randomized trial. J Dent Res. 2010; 89:823–826. [PubMed] [Google Scholar]
29. Arslan S, Zorba YO, Atalay MA, Özcan S, Demirbuğa S, Pala K, Perçin D, Ozer F. Effect of resin infiltrationon surface properties and Streprococcus mutans adhesion to artificial enamel lesions. Dent Mater J. 2014; 34: 25–30. [PubMed] [Google Scholar]
30. Prasada KL, Penta PK, Ramya KM. Spectrophotometric evaluation of white spot lesion treatment using novel resin infiltration material (ICON®) J Conserv Dent. 2018; 21: 531–535. [PMC free article] [PubMed] [Google Scholar]
31. Núbia P, Daniel N, Flavio A, Renato S, Luis M, José L, et al. Enamel microabrasion: An overview of clinical and scientific considerations. World J Clin Cases. 2015; 3:34–41. [PMC free article] [PubMed] [Google Scholar]
32. Taher NM, Alkhamis HA, Dowaidi SM. The influence of resin infiltration system on enamel micro hardness and surface roughness: An in vitro study. Saudi Dent J. 2012; 24: 79–84. [PMC free article] [PubMed] [Google Scholar]
33. El Assal DW, Saafan AM, Moustafa DH, Al-Sayed MA. The effect of combining laser and nanohydroxy-apatite on the surface properties of enamel with initial defects. J Clin Exp Dent. 2018; 10: e425–e430. [PMC free article] [PubMed] [Google Scholar]
34. Abou Neel EA, Aljabo A, Strange A, Ibrahim S, Coathup M, Young AM, et al. Demineralization–remineralization dynamics in teeth and bone. Int J Nanomedicine. 2016; 11: 4743–4763. [PMC free article] [PubMed] [Google Scholar]
Journal of Dentistry
Volume 21, Issue 1 - Serial Number 66
March 2020
Pages 12-17
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