TY - JOUR ID - 41687 TI - Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study JO - Journal of Dentistry JA - DENTJODS LA - en SN - 2345-6485 AU - Rezazadeh, Fahimeh AU - Moshaverinia, Maryam AU - Motamedifar, Mohammad AU - Alyaseri, Montazer AD - Dept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Shiraz HIV/AIDS Research Center, Dept. of Bacteriology and Virology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Students’ Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. Y1 - 2016 PY - 2016 VL - 17 IS - 1 SP - 49 EP - 54 DO - N2 - Statement of the Problem: Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel.Purpose: This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line.Materials and Method: In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco’s Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA.Results: The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera.Conclusion: The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity.Key Words: Aloe vera; Herpes Simplex Virus-1; Herbal Medicine; Antiviral; Cell Culture UR - https://dentjods.sums.ac.ir/article_41687.html L1 - https://dentjods.sums.ac.ir/article_41687_1cfff62a7853be668725bc1326bfb67e.pdf ER -