Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Isolation and Identification of Non- Commensal Pathogenic Bacteria in the Saliva of Patients Candidate for Liver Transplant: A Cross Sectional Study in Shiraz, South of Iran81864588210.30476/dentjods.2019.77854.ENJananGhapanchiDept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0002-1780-7412AbdollahBazarganiDept. of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0001-6430-0596HoomanKhorshidiDept. of Periodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0001-6936-2454MaryamErfaniStudent, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.FahimehRezazadehOral & Dental Disease Research Center, Dept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0003-2840-7630AzitaAzadOral & Dental Disease Research Center, Dept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0002-1073-4134RezaDerafshiDept. of Prosthetics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0002-4786-2965Ahmad HassanKalantariDept. of Prosthetics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.Journal Article20181117<strong>Statement of the Problem: </strong>Liver cirrhosis is the end stage of liver failure. It is mentioned as one of the main etiologies of morbidity and mortality in the world. The human salivary bacteria may induce oral disorders and interact with other body microbiota.<br /> <strong>Purpose:</strong> The aim of the present study is to identify the pathogenic bacteria of non-oral origin from the saliva samples of patients with end stage liver failure.<br /> <strong>Materials and Method: </strong>In this cross-sectional study, the saliva samples of 88 end stage liver disease cases and 84 age- and sex-matched healthy subjects were collected. The samples were cultured using gram staining and API20E Kit.<br /> <strong>Results: </strong>According to the statistical analysis, the total amount of the non-commensal bacteria was significantly higher in chronic liver failure (CLF) group than controls (<em>p </em>= 0.001). There was no significant difference between both groups for the presence of other bacteria (<em>p </em>= 0.001) except for <em>Escherichia coli</em> (<em>E. coli)</em>. <em>E. coli</em> was isolated from the saliva of 15 cases and only 2 controls.<br /> <strong>Conclusion: </strong>Oral cavity may act as a reservoir for enteric bacteria such as <em>E. coli </em>in liver failure patients. Adequate oral and general hygiene might reduce the risk of systemic infection especially in immunocompromised cases.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601The effect of occlusal loading on gingival microleakage of bulk fill composites compared with a conventional composite87944559410.30476/dentjods.2019.77861.0ENRaziehHoseinifarOral and Dental Diseases Research Center, Dept. of Operative Dentistry, School of Dentistry, Kerman University of Medical Sci-ences, Kerman, Iran.0000-0001-6472-7100MaryamMofidiDept. of Operative Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.0000-0002-6009-4331NimaMalekhoseiniDental Student, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.Journal Article20181130<strong>Statement of the Problem:</strong> Bulk fill composites have been introduced over the recent years in order to accelerate the process of tooth restoration by inserting composite in bulk up to 4mm thickness.<br /> <strong>Purpose:</strong> This in vitro study aims to evaluate the effect of occlusal loading on the gingival microleakage of bulk fill composites compared with a conventional composite.<br /> <strong>Materials and Method:</strong> In this experimental study, box only class II cavities with gingival margins placed 1mm below the cemento-enamel junction were prepared on the mesial and distal surfaces of 36 maxillary premolars (72 cavities). The samples were divided into three groups and restored as follows: Group 1 (Tetric N-Ceram, incremental filling), Group 2 (X-tra fill, bulk filling), Group 3 (Tetric N-Ceram Bulk Fill, bulk filling). All restorations were thermocycled for 2000 cycles (5-50̊C), and then half of the samples were subjected to 200,000 cycles of loading. All the specimens were immersed in 0.5% basic fuchsin for 48 hours, and then, sectioned and evaluated for microleakage with a stereomicroscope. Data were analyzed using Kruskal-Wallis, and Mann-Whitney U-tests. <em>p </em> <strong>Results:</strong> There were no significant differences among the gingival microleakage of three composites in both unloaded and loaded groups. Also, no statistically significant difference was found between the microleakage of unloaded and loaded groups in all materials.<br /> <strong>Conclusion:</strong> Occlusal loading did not affect the gingival microleakage of bulk fill composites, and the microleakage of class II cavities restored with the bulk filling technique was similar to that of restored with the incremental technique.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Comparative analysis of P63, Maspin and Matrix Metalloproteinase 2 Expression in Mucoepidermoid Carcinoma and Adenoid Cystic Carcinoma of Salivary Glands951014589910.30476/dentjods.2019.77868.0ENNasimTaghaviDept. of Oral & Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-0334-9385FarzadYazdaniDept. of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.AlirezaAkbarzadeh BaghbanProteomics Research Center, Dept. of Basic Science, School of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000000209611874SoudabehSargolzaeiDept. of Oral & Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.ParisaKardouni KhozestaniDept. of Oral and Maxillofacial Pathology, Dental School, Anzali International Campus, Guilan university of Medical Sciences, Rasht, Iran.0000-0003-1282-8019Journal Article20181208<strong>Statement of the Problem: </strong>The tumor suppressor role of myoepithelial cells and related mechanisms in breast tumors are well understood. However, the role of these cells in salivary glands tumors is debatable.<br /> <strong>Purpose: </strong>The present study was designed to determine the expression of p63, Maspin and Matrix metalloproteinase 2 <strong>(</strong>MMP-2) in mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma (ADCC) of salivary glands due to various cellular differentiation and structure. The association between the expression of these markers and clinicopathologic features and myoepithelial differentiation were also evaluated.<br /> <strong>Materials and Method: </strong>P63, Maspin and MMP-2 expression were immunohistochemically studied in 67 cases including35 cases of MEC and 32 cases of ADCC. The smooth muscle actin (SMA) staining was also applied to confirm the presence of myoepithelial differentiation. Data was analyzed using Chi-square test, Mann-Whitney U test and t-test.<br /> <strong>Results: </strong>The expression of p63 (<em>p </em>= 0.009) and Maspin (<em>p </em>= 0.012) significantly differed between the study groups.P63 positive cells in MEC were negative for SMA staining in contrast to ADCC. Furthermore, the expression of P63 (<em>p </em>= 0.045) and Maspin (<em>p </em>= 0.019) significantly and inversely correlated with histologic grade in ADCC. Likewise, positive significant correlation was detected between histologic grade and expression of P63 (<em>p </em>= 0.018) and MMP-2 (<em>p </em>= 0.003) in MEC samples.<br /> <strong>Conclusion: </strong>Ourfindings showed thatMEC is devoid of myoepithelial cells. The difference in expression of P63 and Maspin in ADCC and MEC highlighted the role and presence of myoepithelial cells in ADCC. Indeed, the high expression of P63 and Maspin in well differentiated ADCCs suggests the tumor suppressor effect of myoepithelial cells. Considering the association between the evaluated markers and histological grade, p63 in both tumors, Maspin in ADCC and MMP-2 in MEC may be efficient predictors of clinical behavior.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Effect of Preoperative Anesthesia Consultation on Decreasing Anxiety in Patients Undergoing Oral and Maxillofacial Surgery1021054557310.30476/dentjods.2019.77883.0ENFahimehAkhlaghiDental Research Center, Research Institute of Dental Sciences, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Oral and Maxillofacial Surgeon, Dept. of Oral and Maxillofacial Surgery, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0003-0352-8831ShahabaldinAziziResident of Oral and Maxillofacial Surgery, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0001-6648-6312BahmanMalekDept. of Anesthesiology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.FarinazMahboubiPower Electrical Engineer, Tehran University, Tehran, Iran.ShahinShamsOral and Maxillofacial Surgeon, Tehran, Iran.MahdiehKarimizadehPediatrician, Khalij Hospital, Bushehr University of Medial Sciences, Bushehr, Iran.0000-0001-6472-9050Journal Article20190112<strong>Statement of the Problem: </strong>Preoperative anxiety is the subject of major concern for many patients.<br /> <strong>Purpose:</strong> The current study aimed at determining the effect of anesthesia consultation on decreasing anxiety in patients undergoing oral and maxillofacial surgery.<br /> <strong>Materials and Method: </strong>This randomized clinical trial was conducted on 250 patients undergoing different maxillofacial surgeries. The data collection instruments included a questionnaire containing the Spielberger state-trait anxiety inventory (STAI) and a researcher-made questionnaire with queries on the demographic characteristics and surgery-related information. Analysis of the data was performed in SPSS, using descriptive and inferential statistics.<br /> <strong>Results:</strong> The findings showed that the majority of patients (38.4%) had moderate anxiety; there was no significant difference between the consultation and control groups in terms of age and gender. Also, the scores of state and trait anxiety were significantly lower in the consultation group, compared with the control group.<br /> <strong>Conclusion:</strong> The present results showed that preoperative anesthetic consultation reduced preoperative anxiety, compared with the control group. Our findings suggest that anesthetic counselling services should be provided for individuals experiencing high levels of stress.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Parental Awareness about Oral Health Preventive Care and its Relation to DMFT Index in Visually Impaired Children1061104590010.30476/dentjods.2019.80995.0ENNarjesAmrollahiDental Research Center, Dept. of Pediatric Dentistry, Dental Research Institute, Isfahan University of Medical sciences, Isfahan, Iran.0000-0002-0197-4697AndishehAminiDental Student, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.MehdiJafarzadehDental Research Center, Dept. of Pediatric Dentistry, Dental Research Institute, Isfahan University of Medical sciences, Isfahan, Iran.0000-0001-6379-7916Journal Article20190130<strong>Statement of the Problem: </strong>Oral health care for children with visual impairment is challenging for health service providers. Gaining information about parental awareness in this regard can be a basis for health planning and use of preventive services.<br /> <strong>Purpose:</strong> The aim of this study was to evaluate parental awareness about the importance of preventive care and its relationship with DMFT index in visually impaired children.<br /> <strong>Materials and Method:</strong> This cross-sectional and descriptive- analytical study was carried out on 50 parents of children with visual impairment referring to schools of the blind children aged 8-14 years in Isfahan in 2017 – 2018. Parental awareness was measured by knowledge questionnaire. DMFT index of first permanent molar was recorded by examination. Data were analyzed using Spearman correlation coefficient, Pearson correlation and T-test in SPSS 22 software. The level of significance was considered less than 0.05.<br /> <strong>Results:</strong> The mean score of parental awareness about significance of preventive care was 68.4±15.5. Mean DMFT in the studied children was 2.40±1.32 and highest score was related to decayed tooth, followed by filled tooth. There was no significant relation between parental knowledge and mean DMFT (<em>p </em>= 0.30), while there was revers relation with number of extracted teeth (<em>p </em>= 0.02) and direct relation with number of filled tooth (<em>p </em>= 0.04).<br /> <strong>Conclusion</strong>: Parental awareness generally did not show significant relationship with DMFT, while by increasing their knowledge the number of missed teeth was decreased and the number of filled teeth was increased in visually impaired children.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601In Vitro Comparative Evaluation of Newly Produced Desensitizer and Chlorhexidine and Gluma on Bond Strength and Bond Longevity of Composite to Dentin1111184589810.30476/dentjods.2019.77756.0ENRezaDavallooDental Sciences Research Center, Dept. of Restorative Dentistry, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.0000-0003-3711-3597MaryamTavangarDental Sciences Research Center, Dept. of Restorative Dentistry, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.000000018156880XHeshmatollahEbrahimiDept. of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.FaridehDarabiDental Sciences Research Center, Dept. of Restorative Dentistry, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.0000_0001_8696_6698ShimaMahmoudiPostgraduate Student of Restorative Dentistry, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.0000-0003-0853-2493Journal Article20180612<strong>Statement of the Problem: </strong>Etching process on dentin can activate matrix metalloproteinase (MMP) which hydrolyze organic matrix of demineralized dentin. Gluma and chlorhexidine could inhibit the activation of MMP.<br /> <strong>Purpose:</strong> The aim of the study was to evaluate the effect of a new desensitizing material consisting of Gluma and chlorhexidine together on the shear bond strength and bond durability of composite restorations.<br /> <strong>Materials and Method: </strong>One hundred and twenty caries-free extracted premolars were sectioned horizontally from one third of the coronal crown to expose flat dentin surface and randomly divided into 4 groups. In the control group no surface treatment was used. In the first group chlorhexidine (CHX) 2%, in the second group, new material (NM) and in third group Gluma Desensitizer (GD) was applied after etching and before bonding(total-etch bonding system). After the bonding process, the composite was placed on the surface of the samples using a cylindrical mold. Then, the shear bond strength of half of the specimens was measured after 24 hours and the other half after 6 months of storage in distilled water and thermocycling. The failure type of specimens was evaluated with a stereomicroscope. Data were analyzed using one way Anova and Tukey's Post Hoc tests in SPSS software.<br /> <strong>Results: </strong> After 6 months, the bond strength decreased in all groups and differences were statistically significant (<em>p </em>= 0.002).The highest SBS was observed after 6 months in the NM group and the GD group (with no significant difference). Mix failure had the highest rate in all groups. <br /> <strong>Conclusion: </strong>It can be concluded that the effect of combination of chlorhexidine and gluma on maintaining the integrity and strength of bond over time is similar to Gluma compound alone and they have better effect than chlorhexidine.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Influence of Parental Exposure to Risk Factors in the Occurrence of Oral Clefts1191264590110.30476/dentjods.2019.77620.0ENSandraAlto EacuteDept. of Public Health, Nursery School, University of Cuiabá, Cuiabá, Brazil.0000-0002-7416-3430Alvaro HenriqueBorgesDept. of Endodontics, Dental School, University of Cuiabá, Cuiabá, Brazil.0000-0001-9510-6542Ana Thereza De Saboia CamposNevesDept. of Pediatric Dentistry, Dental School, University of Cuiabá, Cuiabá, Brazil.AndrezaMaria Fábio AranhaDept. of Pediatric Dentistry, Dental School, University of Cuiabá, Cuiabá, Brazil0000-0003-0014-7937AlexandreMeireles BorbaDept. of Surgery, Dental School, University of Cuiabá, Cuiabá, Brazil.Mariano MartinezEspinosaDept. of Statistics, Federal University of Mato Grosso, Cuiabá, Brazil.Luiz Evaristo RicciVolpatoDept. of Pediatric Dentistry, Dental School, University of Cuiabá, Cuiabá, Brazil0000-0002-2969-1963Journal Article20171112<strong>Statement of the Problem:</strong> Non-syndromic cleft lip and palate are the most frequent craniofacial abnormalities in humans. The genetic, environmental and behavioral factors involved in this malformation must be clarified in different parts of the globe in the view of implementing preventive measures.<br /> <strong>Purpose:</strong> To analyze the influence of parental exposure to risk factors on the occurrence of oral clefts.<br /> <strong>Materials and Method:</strong> A case-control study was conducted with 150 mothers of oral cleft children paired by the children’s gender to 300 mothers of children without congenital anomalies from Mato Grosso, Brazil, for the study of the variables: gender and race/color of the children; parental educational level; age; number of pregnancies; prenatal care; obesity; stress; diabetes; hypertension; use of medications, alcohol and illicit drugs; smoking and exposure to ionizing radiation during the first trimester of pregnancy. The results were analyzed in relation to the chances possibility of each variable for the occurrence of oral cleft through the bivariate and multivariate analysis by applying the model of logistic regression.<br /> <strong>Results:</strong> Passive smoking, obesity, exposure to ionizing radiation and use of antibiotics were associated with the presence of clefts. The use of folic acid and analgesics were identified as preventive factors. The father's low educational level was found as a risk factor, while the black race/color was a preventive factor; nevertheless these variables were not associated in the multivariate analysis.<br /> <strong>Conclusion:</strong> The results reinforce the need to follow the pregnant women, especially in the first trimester of pregnancy, in order to control the identified risk factors.<br /> Clinical Relevance: Knowing the factors associated with the occurrence of oral clefts and those associated with its prevention, it is possible to apply specific health promotion measures during pregnancy, which can result in the reduction of oral clefts’ occurrence.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Characteristics Affecting Oral Pigmentation in Passive Smoker Children1271314557410.30476/dentjods.2019.81785.0ENFahimehRashidi MaybodiDept. of Periodontics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.0000-0002-2853-8801RoyaGhafourifardDept. of Pediatric, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.MinaMohammad-TaheriDentist, Isfahan, Iran.RezaGolvardi YazdiStudent Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.0000-0002-5575-5270Journal Article20190326<strong>Statement of the Problem:</strong> Smoking affects not only smokers themselves, but also the people around them. 700 million children are exposed to second hand tobacco worldwide. One of the adverse effects of being a passive smoker is oral pigmentation.<br /> <strong>Purpose:</strong> Evaluating association between being a passive smoker and oral pigmentation.<br /> <strong>Materials and Method:</strong> This is a historical cohort. 140 healthy children aged from 4 to 10 with the mean age of 6.68±1.60 years old (70 with a smoker parent and 70 without smoker parents) were examined for oral pigmentation. Environmental factors were evaluated by asking the parents to fill a questionnaire. Data were analyzed using Chi-square test, Fisher's exact test, Logistic regression and Spearman scale.<br /> <strong>Results:</strong> There was a meaningful relationship between having a smoker parent and oral pigmentation (P-value=0.0001). spearman correlation showed parents' duration of cigarette smoking and the number of cigarettes per day meaningfully affect the severity of oral pigmentation (R=0.329). The study did not find a statistical relationship between oral pigmentation in passive smoking and sex or house area.<br /> <strong>Conclusion:</strong> Children exposed to secondhand tobacco are at more risk for oral pigmentation. Its severity depends on duration of cigarette smoking and the number of cigarettes per day.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Comparison of Shear Bond Strength of Three Types of Glass Ionomer Cements Containing Hydroxyapatite Nanoparticles to Deep and Superficial Dentin1321404586810.30476/dentjods.2019.77762.0ENFarahnazSharafeddinDept. of Operative Dentistry, Biomaterials Research Center, School of Dentistry, Shiraz University of Medial Sciences, Shiraz, Iran.0000-0003-2547-079XAli AsgharAlaviDept. of Operative Dentistry, Biomaterials Research Center, School of Dentistry, Shiraz University of Medial Sciences, Shiraz, Iran.0000-0001-6425-1974SabaSiabaniPostgraduate Student, Dept. of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0002-2105-4484MinaSafariPostgraduate Student, Dept. of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0003-2765-4699Journal Article20180619<strong>Statement of the Problem:</strong> The clinical success of glass ionomer cement (GIC) restorations depends on the strength of its bonding to dentin, yet the bond strength of nanohydroxyapatite (nHAp) added GIC to dentin needs to be investigated.<br /> <strong>Purpose:</strong> This study aimed to assess if the type of GIC containing nHAp and dentin depth can affect the shear bond strength (SBS).<br /> <strong>Materials and Method:</strong> In this experimental study, 60 freshly extract intact third molars were randomly divided into two main groups of flat occlusal dentin with different cuts as superficial (S): just below the dentinoenamel junction (DEJ) and deep (D): 2mm below DEJ. After conditioning with 20% polyacrylic acid, each group randomly assigned to the tested GIC (n=10) subgroups as 1: Fuji IX Extra+nHAp, 2: Fuji II LC+nHAp and 3: Zirconomer+nHAp. Plastic tubes were placed on the pre-treated surfaces and filled with one of the GIC, then stored in an incubator at 37<sup>o</sup>C under 100% humidity for 24hr. The specimens were thermocycled at5/55 <sup>o</sup>C for 500 cycles and subjected to shear bond strength (SBS) test using a universal testing machine (1 mm/min). The data analyzed by Mann-Whitney and Kruskal-Wallis test (<em>p </em>< 0.05).<br /> <strong>Results: </strong>The means of SBS of Fuji II LC+nHAp was significantly higher than Fuji IX+nHAp and Zirconomer+nHAp both in superficial and deep dentin (<em>p </em>< 0.05). The means of SBS of Fuji IX Extra+nHAp and Zirconomer+nHAp subgroups in superficial dentin were higher than deep dentin, this differences was statistically significant (<em>p </em>= 0.0001 and <em>p </em>= 0.009, respectively).<br /> <strong>Conclusion:</strong> It can conclude that SBS was influenced by type of GIC, and depth of dentin.Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Glypican-3 Expression in Patients with Oral Squamous Cell Carcinoma1411464590210.30476/dentjods.2019.84541.1089ENAzadehAndisheh-TadbirOral and Dental Disease Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0001-5802-0512Amir SaeedGoharianUndergraduate Student, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0001-6507-0091Mohammad AliRanjbarDept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0001-6383-9944Journal Article20191216<strong>Statement of the Problem: </strong>Oral squamous cell carcinoma (OSCC) is a malignant neoplasm that affect the structures or tissues of the mouth. Early diagnosis of these tumors is important to improve the outcome of treatment. Therefore, using pathological techniques based on molecular markers may be useful for optimal diagnosis and treatment. Glypican-3(GPC3) is involved in regulation of cell proliferation and morphogenesis and is abundant during embryogenesis and organogenesis but is limited in most of adult tissues. GPC3 overexpression has a role in carcinogenesis.<br /> <strong>Purpose: </strong>The aim of the present study was to investigate GPC3 expression in the non-neoplastic oral epithelium and oral squamous cell carcinoma.<br /> <strong>Materials and Method</strong>: In this cross-sectional study, 45 patients with OSCC (30 males and 15 females) with a mean age 52.3 of selected from Oral Pathology Department of Shiraz Dental School were enrolled. The control group was consisted of 15 cases of normal oral epithelium. Glypican-3 expression was assessed by using immunohistochemical methods.<br /> <strong>Results:</strong> Non neoplastic tissues were GPC3 negative. Frequency of GPC3 positivity in tumoral tissues was recorded as 73.3% (33 cases) which was significantly higher than non-neoplastic tissues (<em>p </em>< 0.001).<br /> The clinicopathologic features of GPC3 expression demonstrated no association with clinicopathologic parameters except tumor size.<br /> <strong>Conclusion:</strong> GPC3 was over expressed at protein level in oral squamous cell carcinoma, but its potential use for diagnostic and therapeutic purposes required further investigation. Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Unusual Cystic Variant of Calcifying Epithelial Odontogenic Tumor1471524552710.30476/dentjods.2019.77772.ENMalaKambojDept. of Oral Pathology & Microbiology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India0000-0001-5626-2622Achla BhartiYadavDept. of Oral Pathology, D J College of Dental Sciences & Research, Modinagar, India0000-0001-9617-5849AnjaliNarwalDept. of Oral Pathology & Microbiology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India.0000-0001-7468-7886NeerajKumarDept. of Dentistry, GMCH 32, Chandigarh, India.0000-0001-8771-4193Journal Article20180708Calcifying epithelial odontogenic tumor (CEOT) is a rare benign odontogenic neoplasm which is exclusively epithelial in its tissue of origin. Many cases of CEOTs are associated with impacted tooth and simulate dentigerous cyst, radiographically. The histologic features of CEOT are unique, however, among its various histologic subtypes, the cystic variant is a rare and less well understood entity. Our report elucidates a cystic variant of CEOT in the maxilla of a 16-year-old male that presents clinical and radiologic findings conscientious to dentigerous cyst; but histopathological diagnosis came out to be a gold standard in identifying this rare tumor. Herein, the authors describe the clinicopathologic features of this rare entity, highlighting histomorphologic findings along with reviewing of other reported cases. Shiraz University of Medical SciencesJournal of Dentistry2345-648521220200601Hybrid Odontogenic Tumor of Calcifying Odontogenic Cyst and Ameloblastic Fibroma: a Case Report and Review of Literature1531574552810.30476/dentjods.2019.77806.ENNazaninMahdaviDept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-0691-1580NedaKardooni KhoozestaniDept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-8844-0638MahboubeHasheminasabCraniomaxillofacial Research Center, Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0001-7815-7866NikaSoltaniStudent, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-5213-9966Journal Article20180823Calcifying odontogenic cyst (COC) is an uncommon odontogenic lesion that represents less than 2% of all odontogenic cysts and tumors. It usually occurs in incisor and canine areas during the second to fourth decades of life. It can be associated with other lesions like odontoma, Ameloblastic Fibroma, Ameloblastoma, Adenomatoid odontogenic tumors, Odontoameloblastoma, and Odontogenic Myxoma. Ameloblastic fibroma is a truly mixed tumor usually diagnosed within the posterior mandible during the first two decades of life. In the present article, a hybrid odontogenic tumor composed of COC and Ameloblastic Fibroma in a 14-year-old white Persian female is described.