Document Type: Original Article

Authors

1 Dept. of Endodontics, School of Dentistry, AJA University of Medical Sciences, Tehran, Iran.

2 School of Dentistry, AJA University of Medical Sciences, Tehran, Iran.

3 Dept. of Microbiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Abstract

Statement of the problem: Different materials have been used to repair root perforations, the most successful of which is mineral trioxide aggregate (MTA). It is technically difficult to use MTA for perforation repair. Recently, some bio-ceramic sealers such as Endoseal MTA were introduced to repair the perforation site during root filling, which decreases the technical difficulty of this procedure.
Purpose: The aim of this study was to compare the bacterial microleakage of Pro-Root MTA and Endoseal MTA sealer in root perforation repair.
Materials and Methods: This in vitro experimental study evaluated 40 extracted canine teeth. After root canal cleaning and shaping, a root perforation was artificially created at 7 mm below the cementoenamel junction. The teeth were then randomly divided into two experimental groups (n=18) of Pro-Root MTA and Endoseal MTA, and two positive and negative control groups (n=2). Perforation sealing and root canal filling were performed in the two experimental groups according to the manufacturers’ instructions. After sterilization of the whole system with gamma-ray, microleakage was tested using a double-chamber model. Data regarding the presence/absence of microleakage were reported after 35 days. The data were analyzed by SPSS software using the Chi-square test.
Results: There was no significant difference between the two experimental groups regarding bacterial microleakage (P>0.05).
Conclusions: Under the conditions of this study, it can be concluded that the sealing ability of perforation repair with Endoseal MTA Sealer and Pro-Root MTA was comparable.

Keywords

[1]  Ingle JI. A standardized endodontic technique utilizing newly designed instruments and filling materials. Oral Surg Oral Med Oral Pathol. 1961; 14: 83-91.

[2]  American Association of Endodontists. Glossary of endodontic terms. 9th ed. Chicago: American Association of Endodontists; 2016. p. 36.

[3]  Sahebi S, Moazami F, Sadat Shojaee N, Layeghneghad M. Comparison of MTA and CEM Cement Microleakage in Repairing Furcal Perforation, an in vitro Study. J Dent (Shiraz). 2013; 14: 31-36.

[4]  Haghgoo R, Arfa S, Asgary S. Microleakage of CEM cement and ProRoot MTA as furcal perforation repair materials in primary teeth. Iran Endod J. 2013;8:187-190.

[5]  Silveira CM, Sánchez-Ayala A, Lagravère MO, Pilatti GL, Gomes OM. Repair of furcal perforation with mineral trioxide aggregate: long-term follow-up of 2 cases. J Can Dent Assoc. 2008; 74: 729-733.

[6]  Torabinejad M, Watson TF, Pitt Ford TR. Sealing ability of a mineral trioxide aggregate when used as a root end filling material. J Endod. 1993; 19: 591-595.

[7]  Torabinejad M, Higa RK, McKendry DJ, Pitt*Ford TR. Dye leakage of four root end filling materials: effects of blood contamination. J Endod. 1994; 20: 159-163.

[8]  Dastorani M, Modaresi J, Akhavan A, Manshaii M, Ayatollahi F. Evaluation the effect of demineralized bone matrix as a scaffold on pulp revascularization of immature cat teeth. J Mashhad Dent Sch. 2013; 37: 215-220.

[9]  Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--Part III: Clinical applications, drawbacks and mechanism of action. J Endod. 2010; 36: 400-413.

[10]          Torabinejad M, Hong CU, McDonald F, Pitt*Ford TR. Physical and chemical properties of a new root-end filling material. J Endod. 1995; 21: 349-353.

[11]          Holland R, Otobani*Filho JA, Souza V, Nery MJ, Bernabé PF, Dezan*Junior E. Mineral trioxide aggregate repair of lateral root perforations. J Endod. 2001; 27: 281-284.

[12]          Camilleri J. The chemical composition of mineral trioxide aggregate. J Conserv Dent. 2008; 11: 141–143.

[13]          Lim ESPark YBKwon YSShon WJLee KW, Min KS. Physical properties and biocompatibility of an injectable calcium-silicate-based root canal sealer: in vitro and in vivo study. BMC Oral Health. 2015; 15: 129.

[14]          Kakani AK, Veeramachaneni C, Majeti C, Tummala M, Khiyani L. A Review on Perforation Repair Materials. J Clin Diagn Res. 2015; 9: 9-13.

[15]          Main CMirzayan NShabahang STorabinejad M. Repair of root perforations using mineral trioxide aggregate:

a long-term study. J Endod. 2004; 30: 80-83.

[16]          Viola NV, Guerreiro-Tanomaru JM, da*Silva GF, Sasso Cerri E, Tanomaru-Filho M, Cerri PS. Biocompatibility of an experimental MTA sealer implanted in the rat subcutaneous: quantitative and immunohistochemical evaluation. Biomed Mater Res B Appl Biomater. 2012; 100: 1773-1781.

[17]          Lee SJ, Chung J, Na HS, Park EJ, Jeon HJ, Kim HC. Characteristics of novel root-end filling material using epoxy resin and Portland cement. Clin Oral Investig. 2013; 17: 1009-1015.

[18]          Willershausen I, Wolf T, Kasaj A, Weyer V, Willershausen B, Marroguin BB. Influence of a bioceramic root end material and mineral trioxide aggregates on fibroblasts and osteoblasts. Arch Oral Biol. 2013; 58: 1232–1237.

[19]          Gomes BPPinheiro ETSousa ELJacinto RCZaia AA, Ferraz CC, et al. Enterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: 247-253.

[20]          Jafari F, Jafari S. Importance and methodologies of endodontic microleakage studies: A systematic review. J Clin Exp Dent. 2017; 9: 812-819.

[21]          Aminsobhani M, Ghorbanzadeh A, Behnam B, Noushin S, Sholeh G, Assadian H, et al. Coronal microleakage in root canals obturated with lateral compaction, warm vertical compaction and guttaflow system. Int Endod J. 2010; 5: 83-87.

[22]          Pommel L, Camps J. In vitro apical leakage of system B compared with other filling techniques. J Endod. 2001; 27: 449–451.

[23]          Khatib MS, Devarasanahalli SV, Aswathanarayana RM, Das P, Nadig RR. Comparison of the sealing ability of Endocem mineral trioxide aggregate and Endoseal mineral trioxide aggregate as a furcal perforation repair material under the operating microscope: An in vitro study. Endodontology. 2019; 31: 25-28.

[24]          Adl A, Sadat*Shojaee N, Pourhatami N. Evaluation of the dislodgement resistance of a new pozzolan-based cement (EndoSeal MTA) compared to ProRoot MTA and Biodentine in the presence and absence of blood. Scanning. 2019; 3863069.

[25]          De-Deus G, Petruccelli V, Gurgel-Filho E, Coutinho-Filho T. MTA versus Portland cement as repair material for furcal perforations: a laboratory study using a polymicrobial leakage model. Int Endod J. 2006; 39: 293-298.

[26]          Hwang JH, Chung J, Na HS, Park E, Kwak S, Kim HC. Comparison of bacterial leakage resistance of various root canal filling materials and methods: Confocal laser-scanning microscope study. Scanning. 2015; 37: 422-428.