Document Type: Original Article


1 Dept. Oral Medicine, Member of Kerman Dental and oral Diseases Research Center, School of Dentistry, Kerman University of Medical Sciences, Kerman, IRAN

2 Dept. of Microbiology, School of Medicine, Kerman University of Medical Sciences, Kerman, IRAN

3 Dentist

4 Dept. of Operative Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, IRAN


Statement of Problem: Reducing oral microorganisms before the oral and maxillofacial surgery, which are accomplished through the mouth, can play an important role in reducing the occurrence of infections after surgery. One effective method in reducing the number of microorganisms is the use of antiseptic mouthwash.Aim: This study was conducted to compare the antimicrobial and cytotoxicity activity of Chlorhexidne, Irsha, oral B, Biothene, Povidone iodine, Benzidamine and Camomile mouthwashesMethods and Materials: This is an experimental study in which lactobacillus casei, Candida albicans, Streptococcus mutans, Streptococcus viridance and Streptococcus sunguis were cultured. For each bacterium, the concentration of 0.5 MC farland was prepared. Four concentrations (5%, 1%, 0.5%, 0.1%) of mouthwashes were also prepared. Distilled water was used as negative control. Tukey, and One Way ANOVA, in SPSS, version 17, were used to analyze the data. Also, to evaluate the cell cytotoxicity of the Mouthwash, colorimetric MTT-Colorimetric assay ([3-(4,5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide]) was used.Results: The results showed that nystatin was the most effective mouthwash on Candida albicans .   Irsha, povidone iodine and benzidamin had no antifungal effect. Only at concentrations of 0.1%, the mouthwash had no antifungal effectbut in other concentrations it had.  Chlorhexidine had the most antimicrobial effects. The oncentration of 5% Benzidamine and Irsha had a significant effect on HEPG2, MRF, Saos-2 and KB.  The concentration of 5% Povidone iodine mouthwash had a cytotoxicity effect on HEPG2, MRF and J774.A1 and 1% concentration had an impact on Saos-2 cells. Camomile mouthwash was effective on the cell MRF in concentration of 5% and oral B mouthwash was effective on HEPG2 in the same concentration. Chlorhexidine mouthwash had lower cytotoxic effects on all cell lines.Conclusion: The results of this study showed that chlorhexidine mouthwash had better antimicrobial properties and cytotoxicity as compared to others and is recommended as a good mouthwash to be used by patients.Key words: Mouthwash, Antibacterial, Antifungal, Cytotoxicity