Statement of Problem: The most common oral fungal infection, denture induced stomatitis (DIS), is caused by the yeast candida, which is a normal flora of the digestive and vaginal tracts. Various predisposing factors have been associated with denture related stomatitis caused by candida albicans.Purpose: This study aimed to determine the prevalence of DIS and its predisposing factors in a sample of Iranian population admitted to Dental School of Tehran University.Materials and Methods: In this descriptive, analytical, and cross-sectional case series study 141 clinically healthy complete denture wearer patients attending dental school were enrolled through history taking and examin­ation. Data about age, sex, denture age, dental hygiene habits, smoking hab­its and wearing at night were collected. Data analysis was undertaken on a computer, using SPSS 11.5; Phi test and crammers V test statistical methods were used.Results: In a total number of 141 patients, 38.3% showed DIS. There were 40.8% male and 35.7% female. There was no statistical relationship betwe­en age ( p =0.117), sex ( p = 0.531), denture age ( p =0.166), denture hygie­ne habits ( p =0.144), and smoking habits ( p =0.126) and the presence of DIS, but an association between wearing at night and presence of DIS was demonstrated ( p =0.006).Conclusion: It is believed that wearing complete denture at night can incre­ase the prevalence of DIS. Therefore, it is recommend that denture wearers be informed about the effect of dentures on the occurrence of denture induc­ed stomatitis.KEY WORDS: Candidiasis; Complete Denture (CD); Denture Induced Stomatitis (DIS)