Statement of the Problem: Implant-retained overdenture can improve the stability of dentures and prevent bone loss. Overdenture-wearing patients need special hygiene care.Purpose: The aim of this study was to evaluate the effects of various denture cleansers on the retention of Dio orange O-rings.Method and Materials: In this experimental study, 40 Dio orange O-rings were divided into 4 groups (10 O-rings each) and each group was soaked for equivalent of 6 months in the following solutions: 5.25% NaOCl (1:10 dilution), Corega cleanser tabs, Professional cleanser tabs and water (as the control group). After 6 months, O-rings were tested for 2inch/minutes of tensile force. The peak load-to-dislodgement was recorded. Data were imported to SPSS18 and were analyzed by One-Way ANOVA and Tukey HSD test (p≤ 0.05).Results: Denture cleansing solutions have significant effects on the reduction of retentive value of O-rings (p≤ 0.001). Corega tabs caused the reduction of 15.7% (9.91±0.53 N) in the retentive value of O-rings and Professional tabs caused 15% (10.00±0.86 N). NaOCl caused significant decrease (48%) in retentive value of O-rings (6.10±0.91 N in comparison with the control group (11.76±1 N).Conclusion: This in-vitro study demonstrated that the retention of O-rings was affected when soaked in cleansing solutions. NaOCl caused more reduction in retentive value compared to effervescent cleansers and would not be recommended for cleansing O-rings. These results should be interpreted clinically and the role of other factors in the retention of O-rings should be considered in order to recommend the best cleanser for O-ring overdentures.