Document Type : Original Article

Authors

1 Dept. of Endodontic, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Biotechnology Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Dept. of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

10.30476/dentjods.2021.87208.1239

Abstract

Statement of the Problem: Endodontic materials that are placed in direct contact with living tissues should be biocompatible.
Purpose: We aimed to evaluate the cytotoxicity of Nano Fast Cement (NFC) and compare it with ProRoot Mineral Trioxide Aggregate (ProRoot MTA).
Purpose: In vitro assessment of the cytotoxic effects of Nano Fast Cement in comparison to ProRoot MTA on L-929 mouse fibroblast cells.
Materials and Method: In this animal studyL-929 mouse fibroblast cells were grown in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) in an atmosphere of 5% co₂/95% air at 37 C̊. 10⁴ cells from the fourth collection were plated in each well of a 96-well micro-titer plate. Materials were mixed according to the manufacturer’s instruction and placed into the related plastic molds with 5 mm diameter and 3 mm height. After 24 hours and a complete setting, the extracts of the tested materials were produced at six different concentrations and placed in the related wells. Cells in DMEM served as the negative control group. DMEM alone was used as the positive control group. Methyl-thiazoltetrazolium (MTT) colorimetric assay was conducted after 24,48,72 hours. The absorbance values were measured by ELISA plate reader at 540 nm wavelength. Three-way analysis of variance, post-hoc Tukey, LSD, and independent t-test were used for the statistical analyses using SPSS software, version 16.0.
Results: There was no statically significant difference between MTA and NFC in cell viability values at different concentrations and different time intervals (p= 0.649). Viability values were significantly decreased after 72 hours, but there was no significant difference between the first and second MTT assays (p= 0.987). Cytotoxicity significantly increased at concentrations higher than 6.25 µɡ/ml.
Conclusion: Cytotoxicity depends on time, concentration, and cement composition. There was no statically significant difference between NFC and MTA with respect to their cytotoxic effects on L-929 mouse fibroblast cells.

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