Document Type : Original Article

Authors

1 Dept. of Operative, Shahed University of Tehran, Tehran, Iran.

2 Dept. of Operative, Dental School, Ilam University of Medical Sciences, Ilam, Iran.

3 School of Pharmacy, Shahid Beheshti University of Medical Sciences (SBMU) Tehran, Iran.

Abstract

Statement of the Problem: Every effort for increasing the calcium concentration in the saliva would be beneficial for prevention of dental caries. Regarding this issue, the natural products could be considered safer and more cost effective. Sesame is rich in calcium but the data about the effect of sesame on enamel roughness is inadequate.
Purpose: This study aimed to assess the effect of an experimental sesame gel on the surface roughness of human enamel by using atomic force microscopy (AFM).
Materials and Method: In the current experimental study, fifteen enamel slices with 1mm thickness were prepared. They were polished and acid etched to produce a substantial rough surface prior to the first AFM analysis. The enamel blocks were randomly divided into three groups treated with distilled water, fluoride gel, and an experimental prepared sesame gel correspondingly. The treating agent was applied for 3minutes at 0, 8, 24, and 48h intervals and washed by distilled water after each cycles. Ultimately, the final AFM micrographs were prepared. The statistical analysis was performed using paired t-test, one-way ANOVA, and Tukey Post Hoc tests (a=0.05).
Results: Statistical analysis revealed that the surface roughness was significantly reduced in both sesame and fluoride groups (p= 0.017 and 0.018, respectively) while the control group (distilled water) were not noticeably changed (p= 0.12). The control group had statistically significant difference with both the sesame and the fluoride groups (p= 0.007 and 0.007, respectively) while the there was no significance difference between sesame and fluoride groups (p= 0.997). 
Conclusion: Following demineralization by acid etched process, the sesame gel significantly reduced surface roughness of enamel and its effect was similar to fluoride gel.

Keywords

  • Moses J, Rangeeth B, Gurunathan D. Prevalence of dental caries, socio-economic status and treatment needs among 5 to 15 year old school going children of Chidambaram. J Clin Diagn Res. 2011; 5: 146-151.
  • Karlinsey RL, Mackey AC, Walker ER, Frederick KE. Surfactant-modified β-TCP: structure, properties and in vitro remineralization of subsurface enamel lesions. J Mater Sci Mater Med. 2010; 21: 2009-2020.
  • Reynolds E. Calcium phosphate‐based remineralization systems: scientific evidence? Aust Dent J. 2008; 53: 268-273.
  • Peters MC. Strategies for noninvasive demineralized tissue repair. Dent Clin. 2010; 54: 507-525.
  • Cai F, Shen P, Morgan M, Reynolds E. Remineralization of enamel subsurface lesions in situ by sugar‐free lozenges containing casein phosphopeptideamorphous calcium phosphate. Aust Dent J. 2003; 48: 240-243.
  • Karlinsey RL, Mackey AC, Walker ER, Frederick KE. Preparation, characterization and in vitro efficacy of an acid-modified β-TCP material for dental hard-tissue remineralization. Acta Biomater. 2010; 6: 969-978.
  • Manton DJ, Walker GD, Cai F, Cochrane NJ, Shen P, Reynolds EC. Remineralization of enamel subsurface lesions in situ by the use of three commercially available sugar‐free gums. Int J Paediatr Dent. 2008; 18: 284-290.
  • Walker G, Cai F, Shen P, Bailey D, Yuan Y, Cochrane N, et al. Consumption of milk with added casein phosphopeptide‐amorphous calcium phosphate remineralizes enamel subsurface lesions in situ. Aust Dent J. 2009; 54: 245-249.
  • Attin T, Paque F, Ajam F, Lennon A. Review of the current status of tooth whitening with the walking bleach technique. Int Endod J. 2003; 36: 313-329.
  • Gama*Cunha AG, De*Vasconcelos M, Alcantara A, Dutra*Borges BC, De*Oliveira*Vitoriano J, Alves‐Junior C, et al. Efficacy of in‐office bleaching techniques combined with the application of a casein phosphopeptide‐ amorphous calcium phosphate paste at different moments and its influence on enamel surface properties. Microsc Res Tech. 2012; 75: 1019-1025.
  • Aparna S, Srirangarajan S, Malgi V, Setlur KP, Shashidhar R, Setty S, et al. A Comparative evaluation of the antibacterial efficacy of honey in vitro and antiplaque efficacy in a 4‐day plaque regrowth model in vivo: preliminary results. J Periodontol. 2012; 83: 1116-1121.
  • Moussa A, Saad A, Noureddine D, Aboud B, Meslem A, Baghdad K. The influence of starch of ginger on the antibacterial activity of honey of different types from algeria against Escherichia coli and Staphylococcus aureus. Int J Microbiol Res. 2011; 2: 258-262.
  • Moynihan P. Foods and factors that protect against dental caries. Nutr Bull. 2000; 25: 281-286.
  • Kamchan A, Puwastien P, Sirichakwal PP, Kongkachuichai R. In vitro calcium bioavailability of vegetables, legumes and seeds. J Food Compost Anal. 2004; 17: 311-320.
  • Kang MH, Naito M, Tsujihara N, Osawa T. Sesamolin inhibits lipid peroxidation in rat liver and kidney. J Nutr. 1998; 128: 1018-1022.
  • Asokan S, Rathinasamy T, Inbamani N, Menon T, Kumar SS, Emmadi P, et al. Mechanism of oil-pulling therapy- in vitro Indian J Dent Res. 2011; 22: 34.
  • Abebe Y, Bogale A, Hambidge KM, Stoecker BJ, Bailey K, Gibson RS. Phytate, zinc, iron and calcium content of selected raw and prepared foods consumed in rural Sidama, Southern Ethiopia, and implications for bioavailability. J Food Compost Anal. 2007; 20: 16116-8.
  • Saravanan D, Ramkumar S, Vineetha K. Effect of oil pulling with sesame oil on plaque-induced gingivitis: A microbiological study. J Orofac Res. 2013; 3: 175.
  • Thaweboon S, Nakaparksin J, Thaweboon B. Effect of oil-pulling on oral microorganisms in biofilm models. Asia J Public Health. 2011; 2: 62-66.
  • Selwitz RH, Ismail AI, Pitts NB. Dental caries. Lancet. 2007; 369: 51-59.
  • Rezvani MB, Kamali-Nejad M, Karimi M, Raad H, Hamze F. Sesame extraction gel as an agent for prevention of dental caries: An in vitro J Oral Health Oral Epidemiol. 2017; 6: 226-230.
  • Poggio C, Lombardini M, Colombo M, Bianchi S. Impact of two toothpastes on repairing enamel erosion produced by a soft drink: an AFM in vitro J Dent. 2010; 38: 868-874.
  • Chen PR, Chien KL, Su TC, Chang CJ, Liu TL, Cheng H, et al. Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia. Nutr Res. 2005; 25: 559-567.
  • Poneros‐Schneier A, Erdman J. Bioavailability of calcium from sesame seeds, almond powder, whole wheat bread, spinach and nonfat dry milk in rats. J Food Sci. 1989; 54: 150-153.
  • Wang C, Huang S, Liu Y, Li J, Yu H. The CPP-ACP relieved enamel erosion from a carbonated soft beverage: an in vitro AFM and XRD study. Arch Oral Biol. 2014; 59: 277-282.