Evaluation of Mast Cell Density in Mucoepidermoid Carcinoma and Pleomorphic Adenoma

Jalili, Azadeh; Jafari, Najmeh; Tabatabaei, Seyed Hosein

doi:10.30476/dentjods.2022.93712.1730

Evaluation of Mast Cell Density in Mucoepidermoid Carcinoma and Pleomorphic Adenoma

Document Type : Original Article

Authors

Azadeh Jalili ¹

Najmeh Jafari ²

Seyed Hosein Tabatabaei ³

¹ School of Dentistry, Shahid Sadoughi University of Medical sciences, Yazd, Iran.

² Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Sadoughi University of Medical sciences, Yazd, Iran.

³ Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Social Determinant of Oral health Research Center, Shahid Sadoughi University of Medical sciences, Yazd, Iran.

https://doi.org/10.30476/dentjods.2022.93712.1730

Abstract

Statement of the Problem: Mast cells are round to elliptical cells that originate from bone marrow stem cells and enter the peripheral blood. By releasing inflammatory mediators, these cells are involved in type I hypersensitivity, wound healing, defense against pathogens, increased blood vessel formation, and destruction of the extracellular matrix. There are contradictory results regarding the role of mast cells in tumor lesions.
Purpose: Considering the contradictory results and few studies on the density of mast cells in salivary tumors, the present study investigated and compared the density of mast cells in two common salivary gland tumors.
Materials and Method: In the cross-sectional study after reviewing the records of patients referred to the Pathology Department of the School of Dentistry and Shahid Sadoughi Hospital in Yazd, 15 blocks of each of the mucoepidermoid carcinoma and pleomorphic adenoma tumors were taken. After Giemsa staining of the samples, the average of stained cells in 10 random fields under 400× magnification was counted. The results were analyzed using statistical tests of t-test, ANOVA, Chi-square, and Mann-Whitney in SPSS ver. 22.
Results: The average mast cell counts in pleomorphic adenoma (4.2) was higher than muco-epidermoid carcinoma (1.7) but there was no significant relationship (p= 0.305). In mucoepidermoid carcinoma, the numbers of mast cells increased with increasing tumor grade (low: 0/467 moderate: 1/567 high: 2/983) and there was a significant relationship (p= 0.009).
Conclusion: According to the results of the present study, it seems that the mast cells accumulation may be secondarily associated with inflammatory responses due to cell accumulation and tissue destruction by tumor cells.

Keywords

Mast cells

Carcinoma Mucoepidermoid

Adenoma Pleomorphic

Najmeh Jafari Google Scholar

Jaafari-Ashkavandi Z, Ashraf MJ. Increased mast cell counts in benign and malignant salivary gland tumors. J Dent Res Dent Clin Dent Pros. 2014; 8: 15–20.
Mahita V, Manjunatha B, Shah R, Astekar M, Purohit S, Kovvuru S. Quantification and localization of mast cells in periapical lesions. Ann Med Health Sci Res. 2015; 5: 115-118.
Kouhsoltani M, Khiavi MM, Jamali G, Farnia S. Immunohistochemical assessment of mast cells and small blood vessels in dentigerous cyst, odontogenic keratocyst, and periapical cyst. Adv Pharm Bull. 2015; 5 (Suppl 1): 637–641.
Ismerim AB, de*Oliveira*Araújo IB, de Aquino*Xavier FC, Rocha CA, Macedo CL, Cangussu MC, et al. Mast Cells and Proteins Related to Myofibroblast Differentiation (PAR-2, IL-6, and TGFβ1) in Salivary Cancers: A Preliminary Study. Appl Immunohistochem Mol Morphol. 2021; 29: e57-e67.
Seifi S, Yazdani F, Bijani A, Rayat N, Amini*Shakib P. Comparison and evaluation of mast cell density in pleomorphic adenoma, mucoepidermoid carcinoma and adenoid cystic carcinoma of salivary gland with toluidine blue staining. J Babol Univ Med Sci. 2013; 15: 63-69.
Amanpour S, Rad M, Mirshekari TR, Torabi-Parizi M, Fardisi S, Shafazand S. Survey of Mast Cells Density in the Most Prevalent Benign and Malignant Salivary Gland Tumors in Southeast of Iran, 2005-2015. J Rafsanjan Univ Med Sci. 2017; 15: 1027-1038.
Vidal MT, de*Oliveira*Araújo IB, Gurgel CA, Pereira*Fde A, Vilas-Bôas DS, Ramos EA, et al. Density of mast cells and microvessels in minor salivary gland tumors. Tumour Biol. 2013; 34: 309-316.
Yazdani F, Rezvani G, Safari S, Jaberi M. Histopathological comparision of mast cell density in various grades of mucoepidermoid carcinoma. Cancer Res J. 2015; 3: 100-103.
Katopodi E, Kavantzas N, Pavlopoulos P, Papanikolaou V, Saetta A, Korkolopoulou P, et al. The frequency and distribution of mast cells in pleomorphic adenomas of salivary glands. Pathology. 2004; 36: 258-261.
Ashkavandi Z, Jaafari-Moshref M, Mashhadi-Abbas F, Sargolzaie S, Taghavi N. Evaluation of CD31 expression and mast cell count in dysplastic lesions and squamous cell carcinoma of the oral cavity. Iranian Red Crescent Med J. 2010; 12: 272-276.
Ranieri G, Labriola A, Achille G, Florio G, Zito AF, Grammatica L, et al. Microvessel density, mast cell density and thymidine phosphorylase expression in oral squamous carcinoma. Int J Onco. 2002; 21: 1317-1323.
Coussens LM, Raymond WW, Bergers G, Laig-Webster M, Behrendtsen O, Werb Z, et al. Inflammatory mast cells up-regulate angiogenesis during squamous epithelial carcinogenesis. Genes Dev. 1999; 13: 1382-1397.
Kabiraj A, Jaiswal R, Singh A, Gupta J, Singh A, Samadi FM. Immunohistochemical evaluation of tumor angiogenesis and the role of mast cells in oral squamous cell carcinoma. J Cancer Res Ther. 2018; 14: 495-502.
Fukushima H, Ohsawa M, Ikura Y, Naruko T, Sugama Y, Suekane T, et al. Mast cells in diffuse large B‐cell lymphoma; their role in fibrosis. Histopathology. 2006; 49: 498-505.
Ribatti D, Ennas MG, Vacca A, Ferreli F, Nico B, Orru S, et al. Tumor vascularity and tryptase‐positive mast cells correlate with a poor prognosis in melanoma. Eur J Clin Invest. 2003; 33: 420-425.
Nonomura N, Takayama H, Nishimura K, Oka D, Nakai Y, Shiba M, et al. Decreased number of mast cells infiltrating into needle biopsy specimens leads to a better prognosis of prostate cancer. British J Cancer. 2007; 97: 952-956.
Rajput AB, Turbin DA, Cheang MC, Voduc DK, Leung S, Gelmon KA, et al. Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases. Breast Cancer Res Treat. 2008; 107: 249-57.
Gomes AP, Johann JE, Lovato GG, Ferreira AM. Comparative analysis of the mast cell density in normal oral mucosa, actinic cheilitis and lip squamous cell carcinoma. Brazilian Dent J. 2008; 19: 186-189.
Mohtasham N, Babakoohi S, Salehinejad J, Montaser-Kouhsari L, Shakeri MT, Shojaee S, et al. Mast cell density and angiogenesis in oral dysplastic epithelium and low- and high-grade oral squamous cell carcinoma. Acta Odontol Scand. 2010; 68: 300-304.
Naik R, Pai MR, Poornima*Baliga B, Nayak KS, Shankarnarayana, Dighe P. Mast cell profile in uterine cervix. Indian J Patho Micro. 2004; 47: 178-180.
Sharma B, Sriram G, Saraswathi TR, Sivapathasundharam B. Immunohistochemical evaluation of mast cells and angiogenesis in oral squamous cell carcinoma. Indian J Dent Res. 2010; 21: 260-265.
Theoharides TC, Conti P. Mast cells: the Jekyll and Hyde of tumor growth. Trends Immunol. 2004; 25: 235-241.
Tadbir AA, Pardis S, Ashkavandi ZJ, Najvani AD, Ashraf MJ, Taheri A, et al. Expression of Ki67 and CD105 as proliferation and angiogenesis markers in salivary gland tumors. Asian Pac J Cancer Prev. 2012; 13: 5155-5159.
Moghadam SA, Abadi AM, Mokhtari S. Immunohistochemical analysis of CD34 expression in salivary gland tumors. J Oral Maxillofac Patho (JOMFP). 2015; 19: 30–33.
Ota T, Ota K, Jono H, Fujimori H, Ueda M, Shinriki S, et al. Midkine expression in malignant salivary gland tumors and its role in tumor angiogenesis. Oral Oncology. 2010; 46: 657-661.
Ammendola M, Sacco R, Sammarco G, Luposella M, Patruno R, Gadaleta CD, et al. Mast cell-targeted strategies in cancer therapy. Transfus Med Hemother. 2016; 43: 109-113.
Patruno R, Marech I, Zizzo N, Ammendola M, Nardulli P, Gadaleta C, et al. c-Kit expression, angiogenesis, and grading in canine mast cell tumour: a unique model to study c-Kit driven human malignancies. BioMed Res Int. 2014; 2014: 730246.
Josephs DH, Fisher DS, Spicer J, Flanagan RJ. Clinical pharmacokinetics of tyrosine kinase inhibitors: implications for therapeutic drug monitoring. Ther Drug Monit. 2013; 35: 562-587.
Marech I, Gadaleta CD, Ranieri G. Possible prognostic and therapeutic significance of c-Kit expression, mast cell count and microvessel density in renal cell carcinoma. Inte J Molecular Sci. 2014; 15: 13060-13076.
Arock M, Sotlar K, Akin C, Broesby-Olsen S, Hoermann G, Escribano L, et al. KIT mutation analysis in mast cell neoplasms: recommendations of the European Competence Network on Mastocytosis. Leukemia. 2015; 29: 1223-1232.
Ammendola M, Leporini C, Marech I, Gadaleta CD, Scognamillo G, Sacco R, et al. Targeting mast cells tryptase in tumor microenvironment: a potential antiangiogenetic strategy. BioMed Res Int. 2014; 2014: 154702.