Document Type : Original Article


1 Dept. of Pediatrics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.

2 Dept. of Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Postgraduated Student, Dept. of Endodontics, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.

4 General Dentist, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.

5 Dept. of ENT and Head and Neck, Firoozgar Hospital, School of Medicine, ENT and Head and Neck Research Center, Iran University of Medical Sciences, Tehran, Iran.



Statement of the Problem: Success of pulpotomy of primary teeth depends on biological and cytotoxic effects of pulp capping agents. Mineral trioxide aggregate (MTA), Biodentine, calcium enriched mixture (CEM) cement, and ferric sulfate (FS) are among the commonly used pulp capping agents (PCAs) for pulpotomy, and their successful application has been previously evaluated.
Purpose: This study aimed to compare the cytotoxicity of PCAs against mesenchymal stem cells isolated from human exfoliated deciduous teeth (SHEDs).
Materials and Method: In this in vitro study, SHEDs were exposed to MTA, Biodentine, CEM cement, and FS for 24 and 72 hours. The methyl thiazolyl tetrazolium (MTT) assay was performed for five different concentrations of PCAs after 24 and 72 hours of exposure. Data were analyzed by ANOVA.
Results: Generally, the biocompatibility increased by reduction in concentration. All tested concentrations showed higher biocompatibility at 72 hours compared with 24 hours (p< 0.0001). Comparison of cytotoxicity of different biomaterials revealed no significant difference at any time point (p> 0.05).
Conclusion: In general, the cytotoxicity of MTA, Biodentine, CEM cement, and FS was comparable, with no significant difference. Cytotoxicity decreased over time and by a reduction in concentration of biomaterials. MTA and Biodentine showed maximum biocompatibility followed by FS, and CEM cement.