Document Type : Original Article
Authors
- Leila Jabbareh 1
- Hodis Ehsani 1
- Hamidreza Goli 2
- Abolfazl Hossein Nattaj 3
- Sajjad Ebadian 4
- Melika Mollaei 5
- Tahura Etezadi 6
1 Dept. of Periodontics, Dental Research Center, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
2 Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
3 Dept. of Biostatistics, Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran.
4 Dentist, Dental Research Center, Student Research Committee, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
5 Dentistry Student, Dental Research Center, Student Research Committee, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
6 Dept. of Orthodontics, Dental Research Center, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract
Background: The presence of orthodontic appliances in the oral cavity increases the number of cariogenic microorganisms, highlighting the risk of periodontal disease and dental caries. Many approaches can be employed to overcome this problem.
Purpose: This study was conducted to compare the effect of kefir drink with chlorhexidine (CHX) mouthwash on the growth of oral bacteria in patients undergoing orthodontic treatment.
Materials and Method: In this single-blind clinical trial study, 30 candidates for orthodontic treatment were selected and randomly divided into two groups (N=15). The intervention group received 100 ml of Kefir drink twice daily, while the control group used 10 ml of 0.2% mouthwash for 20 days. Microbial sampling was done three times including before intervention, immediately after intervention, and 20 days after intervention. Data were analyzed using SPSS software V.23 with a significance level of less than 0.05.
Results: The findings suggested that the number of bacteria in the CHX group was significantly higher than in the intervention group (p= 0.003). However, no significant difference was observed between the two groups after 20 days (p= 0.148). Furthermore, the number of bacteria decreased significantly in both groups over time.
Conclusion: Both CHX and Kefir have antibacterial properties against oral bacteria.
Highlights
Leila Jabbareh (PubMed)
Tahura Etezadi (Google Scholar)
Keywords
- Santonocito S, Polizzi A. Oral microbiota changes during orthodontic treatment. Front Biosci. 2022; 14: 19.
- Kamran MA, Qasim M, Udeabor SE, Hameed MS, Mannakandath ML, Alshahrani I. Impact of riboflavin mediated photodynamic disinfection around fixed orthodontic system infected with oral bacteria. Photodiagnosis Photodyn Ther. 2021; 34: 102232.
- Akamp T, Rosendahl A, Galler KM, Wölflick M, Buchalla W, Widbiller M. An in vitro coculture approach to study the interplay between dental pulp cells and Streptococcus mutans. Int Endod J. 2024; 57: 164-177.
- Motallaei MN, Yazdanian M, Tebyanian H, Tahmasebi E, Alam M, Abbasi K, et al. The current strategies in controlling oral diseases by herbal and chemical materials. Evid Based Complement Alternat Med. 2021; 21; 3423001.
- Baffone W, Sorgente G, Campana R, Patrone V, Sisti D, Falcioni T. Comparative effect of chlorhexidine and some mouthrinses on bacterial biofilm formation on titanium surface. Curr Microbiol. 2011; 62: 445-451.
- Huang S, Wu M, Li Y, Du J, Chen S, Jiang S, et al. The dlt operon contributes to the resistance to chlorhexidine in Streptococcus mutans. Int J Antimicrob Agents. 2022; 59: 106540.
- Moreira AC, Santos TD, Carneiro MC, Porto MR. Activity of a mouthrinse containing chlorhexidine at 0.12% against the saccharolytic microbiota of saliva. J Biomed Sci. 2008; 7: 266-272.
- Jose JE, Padmanabhan S, Chitharanjan AB. Systemic consumption of probiotic curd and use of probiotic tooth paste to reduce Streptococcus mutans in plaque around orthodontic brackets. Am J Orthod Dentofacial Orthop. 2013; 144: 67-72.
- Parichat P, Pongsak R. Probiotics: Sources, selection and health benefits. Res J Biotechnol. 2023; 18: 5.
- Bustamante M, Oomah BD, Mosi-Roa Y, Rubilar M, Burgos-Díaz C. Probiotics as an adjunct therapy for the treatment of halitosis, dental caries and periodontitis. Probiotics Antimicrob Proteins. 2020; 12: 325-334.
- Ghasempour M, Sefidgar SAA, Moghadamnia AA, Ghadimi R, Gharekhani S, Shirkhani L. Comparative study of Kefir yogurt-drink and sodium fluoride mouth rinse on salivary mutans streptococci. J Contemp Dent Pract. 2014; 15: 214.
- Knop LAH, Shintcovsk RL, Retamoso LB, Grégio AMT, Tanaka O. The action of corticosteroids on orthodontic tooth movement: a literature review. Dental Press J Orthod. 2012; 17: 20e1-e5.
- Salehi P, Sh MD. Comparison of the antibacterial effects of persica mouthwash with chlorhexidine on streptococcus mutans in orthodontic patients. Daru J Pharm Sci. 2006; 14: 178-182.
- Alp S, Baka ZM. Effects of probiotics on salivary Streptecoccus mutans and Lactobacillus levels in orthodontic patients. Am J Orthod Dentofacial Orthop. 2018; 154: 517-523.
- Deus FP, Ouanounou A. Chlorhexidine in dentistry: pharmacology, uses, and adverse effects. Int Dent J. 2022; 72: 269-277.
- Meroni G, Panelli S, Zuccotti G, Bandi C, Drago L, Pistone D. Probiotics as therapeutic tools against pathogenic biofilms: have we found the perfect weapon? Microbiol Res. 2021; 12: 916-937.
- Shah SS, Nambiar S, Kamath D, Suman E, Unnikrishnan B, Desai A, et al. Comparative evaluation of plaque inhibitory and antimicrobial efficacy of probiotic and chlorhexidine oral rinses in orthodontic patients: a randomized clinical trial. Int J Dent. 2019; 2019:
- Widyarman AS, Yunita ST, Prasetyadi T. Consumption of yogurt containing probiotic Bifidobacterium lactis reduces Streptococcus mutans in orthodontic patients. Sci Dent J. 2018; 2: 19-25.
- Lindquist B, Emilson C. Distribution and prevalence of mutans streptococci in the human dentition. J Dent Res. 1990; 69: 1160-1166.
- Loesche W, Rowan J, Straffon L, Loos P. Association of Streptococcus mutans with human dental decay. Infect Immun. 1975; 11: 1252-1260.
- Sullivan A, Borgström MK, Granath L, Nilsson G. Number of mutans streptococci or lactobacilli in a total dental plaque sample does not explain the variation in caries better than the numbers in stimulated whole saliva. Community Dent Oral Epidemiol. 1996; 24: 159-163.
- Lindquist B, Emilson C. Distribution and prevalence of mutans streptococci in the human dentition. J Dent Res. 1990; 69: 1160-1166.
- Näse L, Hatakka K, Savilahti E, Saxelin M, Pönkä A, Poussa T, et al. Effect of long–term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001; 35: 412-420.
- Cogulu D, Topaloglu-Ak A, Caglar E, Sandalli N, Karagozlu C, Ersin N, et al. Potential effects of a multis-train probiotic-kefir on salivary Streptococcus mutans and Lactobacillus spp. J Dent Sci. 2010; 5: 144-149.
- Ghasempour M, Sefidgar A, Gharekhani S, Shirkhani L, Moghadamnia A. Comparison of the effect of probiotic yogurt-drink kefir,% 0.2 chlorhexidine and% 0.2 sodium fluoride mouthwashes on streptococcus mutans: An In vitro study. J Babol Univ Med Sci. 2013; 15: 12-18.
- Hamdi K, Shoa HA, Ordouzadeh N, Ghaemi A. The effect of black and green-tea extracts on dental-plaque forming Streptococci. J Shahrekord Univ Med Sci. 2008; 10: 1-8.