Document Type : Original Article
Authors
1 Orthodontics Resident, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
2 Dept. of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Abstract
Background: Relapse is acknowledged as a substantial failure after orthodontic correction. As a biomarker of bone formation, alkaline phosphatase (ALP) levels will decline in compressive sites during relapse subsequent to orthodontic tooth displacement. Manipulating alveolar bone remodeling to elevate ALP levels using novel biomaterial is presently regarded as one of the innovative approaches to avert relapse effectively.
Purpose: This study aimed to evaluate the effect of carbonated hydroxyapatite (CHA)-statin nanoemulsion on ALP levels in relapsed rats.
Materials and Method: In this in vivo study, forty-eight (n= 48) rats were divided into four groups: control, CHA, statin, and CHA-statin, with 12 rats in each group. A 30g mesial tract-ion was applied for seven days using a closed-coil spring extending from the first maxillary molar to the maxillary incisor. Over seven days, CHA, statin hydrogel, and nanoemulsion CHA-statin were injected every three days to preserve the repositioned teeth. Removing the appliances allowed for relapse to occur. ALP levels were assessed using an enzyme-linked immunosorbent assay on days 0, 1, 7, and 14 after debonding. The acquired data was analyzed using analysis of variance and a post hoc Tukey's test with a significance level of p< 0.05.
Results: The average ALP levels between the groups did not differ significantly (p> 0.05) on days 0 and 1 of relapse. The mean values were highest in the CHA-statin nanoemulsion group. The control group exhibited the lowest average ALP levels on day 7 of the relapse phase, followed by Group CHA, Group statin (St), and Group CHA-St, with a significant difference (p< 0.05). On day 14, the CHA-St group had the highest average ALP levels (p< 0.05), while the CHA and St groups did not differ (p> 0.05).
Conclusion: The results indicated that nanoemulsion CHA-statin could elevate ALP levels during orthodontic relapse.
Highlights
Ananto Ali Alhasyimi (Google Scholar)
Keywords
- Yazdanpanahi F, Shahi M, Vossoughi M, Davaridolatabadi N. Investigating the effective factors on the acceptance of teleorthodontic technology based on the technology acceptance model 3 (TAM3). J Dent Shiraz Univ Med Sci. 2024; 25: 68-76.
- Hadi AFN, Aghniya SN, Haidar GA, Sihombing WSM, Sutedjo A, Alhasyimi AA. Post-orthodontic relapse prevention through administration of a novel synthetic carbonated hydroxyapatite-chitosan hydrogel derived from blood cockle shell (Anadara granosa L.). Dent J (Basel). 2024; 12: 18.
- Alhasyimi AA, Suparwitri S, Christnawati C. Effect of carbonate apatite hydrogel-advanced platelet-rich fibrin injection on osteoblastogenesis during orthodontic relapse in rabbits. Eur J Dent. 2021; 15: 412-419.
- Seifi M, Ezzati B, Saedi S, Mehdi Hedayati M. The effect of ovariectomy and orchiectomy on orthodontic tooth movement and root resorption in wistar rats. Dent Shiraz Univ Med Sci. 2015; 16: 302-309.
- Alhasyimi AA, Pudyani PS, Asmara W, Ana ID. Enhancement of post-orthodontic tooth stability by carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin in rats. Orthod Craniofac Res. 2018; 21: 112–118.
- Nicks KM, Perrien DS, Akel NS, Suva LJ, Gaddy D. Regulation of osteoblastogenesis and osteoclastogenesis by the other reproductive hormones, Activin and Inhibin. Mol Cell Endocrinol. 2009; 310: 11-20.
- Farahani M, Safavi SM, Dianat O, Khoramian Tusi S, Younessian F. Acid and alkaline phosphatase levels in GCF during orthodontic tooth movement. J Dent Shiraz Univ Med Sci. 2015; 16: 237-245.
- Perinetti G, Paolantonio M, D'Attilio M. Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 2002; 122: 548-556.
- Pratt MC, Kluemper GT, Lindstrom AF. Patient compliance with orthodontic retainers in the postretention phase. Am J Orthod Dentofacial Orthop. 2011; 140: 196-201.
- Kartal Y, Kaya B. Fixed orthodontic retainers: a review. Turk J Orthod. 2019; 32: 110–114.
- Woo SB, Hellstein JW, Kalmar JR. Narrative review: bisphosphonates and osteonecrosis of the jaws. Ann Intern Med. 2006; 144: 753-761.
- Fujioka-Kobayashi M, Tsuru K, Nagai H, Fujisawa K, Kudoh T, Ohe G, et al. Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering. J Tissue Eng Regen Med. 2018; 12: 2077–2087.
- Alhasyimi AA, Rosyida NF, Ana ID. Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats. J Oral Biol Craniofac Res. 2025; 15: 129-135.
- Edwards CJ, Russell RG, Spector TD. Statins and bone: myth or reality? Calcif Tissue Int. 2001; 69: 63-66.
- Han G, Chen Y, Hou J, Liu C, Chen C, Zhuang J, et al. Effects of simvastatin on relapse and remodeling of periodontal tissues after tooth movement in rats. Am J Orthod Dentofacial Orthop. 2010; 138: 550.e1-e7:
- Guthrie RM. How safe is aggressive statin therapy? Prog Cardiovasc Nurs 2006; 21: 140-145.
- Matsuyama K, Nakagawa K, Nakai A, Konishi Y, Nishikata M, Tanaka H, et al. Evaluation of myopathy risk for HMG-CoA reductase inhibitors by urethane infusion method. Biol Pharm Bull. 2002; 25: 346-350.
- Oryan A, Kamali A, Moshiri A. Potential mechanisms and applications of statins on osteogenesis: Current modalities, conflicts and future directions. J Control Release. 2015; 215: 12-24.
- Amoozegar H, Ghaffari A, Keramati M, Ahmadi S, Dizaji S, Moayer F, et al. A novel formulation of simvastatin nanoemulsion gel for infected wound therapy: In vitro and in vivo assessment. J Drug Deliv Sci Technol. 2022; 72: 103369.s
- Tada S, Chowdhury EH, Cho C, Akaike T. pH-sensitive carbonate apatite as an intracellular protein transporter. Biomaterials. 2010; 31: 1453–1459.
- Inbarajan ASM, Mathew Punnoose A, Gvv G, Anusha D, David JJ. Effects of Simvastatin on Gene Expression and Alkaline Phosphatase Activity in the MG-63 Cell Line Treated With Hyperglycemia for Bone Regeneration. Cureus. 2024; 16:e55482.
- Oryan A, Kamali A, Moshiri A. Potential mechanisms and applications of statins on osteogenesis: Current modalities, conflicts and future directions. J Control Release. 2015; 215: 12-24.
- Hendrijantini N, Hartono P, Ari MDA, Rantan FA. Human umbilical cord mesenchymal stem-cell therapy to increase the density of osteoporotic mandibular bone. Eur J Dent. 2019; 13: 58-63.
- King GJ, Latta L, Rutenberg J, Ossi A, Keeling SD. Alveolar bone turnover and tooth movement in male rats after removal of orthodontic appliances. Am J Orthod Dentofacial Orthop. 1997; 111: 266–275.
- Maltha JC, Kuijpers-Jagtman AM, Von den Hoff JW, Ongkosuwito EM. Relapse revisited: Animal studies and its translational application to the orthodontic office. Semin Orthod. 2017; 23: 390-398.
- Shetty SV, Patil AK, Ganeshkar SV. Assessment of acid phosphatase and alkaline phosphatase in gingival crevicular fluid in growing and adult orthodontic patients: An in vivo study. J Indian Orthod Soc. 2015; 49: 10–14.
- Arianda TA, Rezqita P, Pudyani PS, Rosyida NF, Alhasyimi AA. Effect of cocoa administration on osteoblast counts and alkaline phosphatase levels during orthodontic tooth movement in rats. J Orofac Sci. 2020; 12: 101–106.
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