Document Type : Original Article
Authors
1 Dept. of Oral and Maxillofacial Pathology, Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
2 Dept. of Immunology and Shiraz Institute for Cancer Researches, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3 Dept. of Pharmacognosy, School of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran.
4 Student Research Committee, Shiraz Institute for Cancer Research, School of Medicine, Shiraz, University of Medical Sciences, Shiraz, Iran.
5 Dept. of Oral & Maxillofacial pathology, Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
Background: The cytotoxic properties of isolated natural compounds from herbal medicines have been revealed in different cancer cell lines.
Purpose: The aim of this study was evaluating cytotoxic and apoptotic effects of Eucalyptus camaldulensis Dehnh. leaf extract on KB human carcinoma cell line.
Materials and Method: In this experimental study, an ethyl acetate extract of dried leaves of Eucalyptus camaldulensis was prepared and dried via a speed vacuum apparatus at 40°C. KB cells were treated with 0, 0.25, 0.5, 0.75 and 1 mg/mL extract. Cisplatin was used as the positive control. MTT assay and Annexin V/7-AAD staining were used to evaluate the cytotoxic and apoptotic effects.
Results: The IC50 values for the Eucalyptus camaldulensis extract were 0.75±0.07 mg/mL and 0.56±0.05 mg/mL at 24 and 48 hours, respectively. Apoptosis increased after 24 hours in a dose-dependent manner and a higher necrotic/late apoptotic population was detected after 48 h.
Conclusion: The ethyl acetate extract of Eucalyptus camaldulensis inhibited KB cell growth via the induction of early apoptosis and late apoptosis; therefore, it might be a potential anticancer agent. The identification and purification of active components are suggested to achieve more conclusive results.
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