Document Type : Original Article

Authors

1 Dept. of Endodontics, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Dental Materials Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Dept. of Community Oral Health, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Dept. of Endodontics, Faculty of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran.

10.30476/dentjods.2026.107163.2724

Abstract

Background: One of the key factors in evaluating the success rate of root canal treatment is apical sealing ability. Studies have shown that all sealers may permit some degree of microleakage. Therefore, in vitro evaluation of microleakage is essential before the clinical application of any new sealer.
Purpose: This study was conducted to assess the microleakage of a novel zinc oxide nano-particle sealer and to compare its performance with a standard sealer (AH26) and a sealer with a similar base but without nano-particles (Pulpdent root canal sealer).
Materials and Method: In this in vitro study, 55 human single-rooted mandibular premolars were selected. The specimens were decoronated at the cemento‑enamel junction, so that all the root samples were standardized to a length of 15 mm. Root canals were prepared using rotary instruments. The specimens were randomly divided in to three groups (AH26, Pulpdent, and new nano zinc oxide-eugenol based sealer, each group containing fifteen specimens). Obturation was performed by cold lateral condensation technique with gutta-percha and the designated sealers in each group. After one week, all teeth underwent fluid filtration test and a second examination was performed 30 days after root canal obturation. Non-parametric statistical analyses were applied. Kruskal Wallis test was used to compare the microleakage between experimental groups and Wilcoxon test was used to compare each group's microleakage over time.
Results: The new nano zinc oxide-eugenol based sealer presented the lowest microleakage (p Value<0.05). The greatest reduction in microleakage between the two measurement intervals (7 days and 30 days), was observed in the AH26 group. The new nano zinc oxide-eugenol based sealer group showed the second highest reduction, which was also statistically significant. The Pulpdent sealer group demonstrated the lowest microleakage reduction between the two measurement intervals, which was not statistically significant.
Conclusion: In the present study, the new nano zinc oxide-eugenol based sealer demonstrated a lower level of microleakage compared with the other similar sealers.

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