Document Type : Original Article
Authors
1 Dept. of Endodontics, School of Dentistry, AJA University of Medical Sciences, Tehran, Iran.
2 Dentist, Student Research Committee, Aja University of Medical sciences, Tehran, Iran.
3 Postgraduate Student, Dept. of Endodontics, School of Dentistry, AJA University of Medical Sciences, Tehran, Iran.
Abstract
Background: In cases where microbial infection is present, reducing the bacterial load within the root canal system is a critical factor for successful root canal treatment. In such situations, the antibacterial properties of root canal sealers may further contribute to improved endodontic outcomes. Formaldehyde (FA) is an effective antimicrobial agent, and its release from resin-based endodontic sealers has been well documented. This release contributes to the antibacterial properties of some endodontic sealers.
Purpose: This study aimed to investigate and compare the antibacterial properties and FA release rate of the Iranian BETA RCS sealer with the common sealers ADSEAL and AH26.
Materials and Method: In this fundamental experimental study, three sealers of BETA RCS, ADSEAL, and AH26 were evaluated. To measure FA release, 6 samples of each AH26 and BETA RCS sealers were prepared by mixing 0.2 g of liquid with the specified powder ratio, and 6 ADSEAL samples were performed by combining 0.2 g of base with the appropriate catalyst ratio, following the manufacturer’s instructions. The samples were then analyzed at 3 time points of (time zero), 48 h, and 1 week. To investigate the antimicrobial properties of the sealers, Enterococcus faecalis and Streptococcus mutans were employed. The samples cultured in Plate Count Agar - PCA medium, incubated at 37 oC for 24 h and the formed-colonies were counted.
Results: The obtained results revealed that AH 26, ADSEAL, and BETA RCS sealers released FA, and the highest amount at all three times was related to AH26 sealer, while the lowest amount was correlated with ADSEAL. In all three sealers, the highest amount of FA release occurred after 48 h. The greatest antibacterial effect on Enterococcus faecalis and Streptococcus mutans was associated with AH26, followed by ADSEAL and BETA RCS, respectively.
Conclusion: Regarding the obtained outcomes, AH26 had the highest amount of FA released. Additionally, the anti-bacterial activity of AH26 demonstrated a stronger effect compared to the other two sealers, with ADSEAL showing acceptable results and BETA RCS exhibited the least antibacterial efficacy.
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